Anti-KLF5 Antibody (C-term)

Product Name

Anti-KLF5 Antibody (C-term)

View all KLF5 Antibodies

SKU/Catalog Number

A00727

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-KLF5 Antibody (C-term) (Catalog # A00727). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-KLF5 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00727)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This KLF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human KLF5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00727 is reactive to KLF5 in Human

Applications

A00727 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.792kDa

Background of KLF5

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50

Validation Images & Assay Conditions

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Fluorescent confocal image of U251 cell stained with KLF5 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.

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KLF5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).

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KLF5 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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KLF5 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For KLF5 (Source: Uniprot.org, NCBI)

Alternative Names

Basic transcription element-binding protein 2; BTEB2; BTE-binding protein 2; CKLFGC-box-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; IKLFbasic transcription element binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; KLF5; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); Transcription factor BTEB2 KLF5 BTEB2, CKLF, IKLF Kruppel like factor 5 Krueppel-like factor 5|(intestinal Kruppel-like factor|BTE-binding protein 2|GC box binding protein 2|Kruppel-like factor 5 (intestinal)|basic transcription element binding protein 2|colon krueppel-like factor|colon kruppel-like factor|epididymis secretory sperm binding protein|intestinal-enriched krueppel-like factor|intestinal-enriched kruppel-like factor|transcription factor BTEB2

For more info on KLF5, check out the KLF5 Infographic

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In this infographic, you will see the following information for KLF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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