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Product Info Summary
| SKU: | A12505 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-KLHL13 Monoclonal Antibody
SKU/Catalog Number
A12505
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KLHL13 Monoclonal Antibody catalog # A12505. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLHL13 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12505)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8D1
Isotype
IgG
Immunogen
Purified recombinant fragment of human KLHL13 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12505 is reactive to Klhl13 in Human
Applications
A12505 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73.787kDa
Background of Klhl13
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400
Validation Images & Assay Conditions
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Western Blot (WB) analysis using KLHL13 Monoclonal antibody against HeLa (1) and MCF-7 (2) cell lysate.
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Immunofluorescence (IF) analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry (IHC) analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) with DAB staining using KLHL13 Monoclonal antibody.
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Flow cytometric (FCM) analysis of 3T3/L1 cells using KLHL13 Monoclonal antibody (green) and negative control (purple).
Protein Target Info & Infographic
Gene/Protein Information For Klhl13 (Source: Uniprot.org, NCBI)
Gene Name
Klhl13
Full Name
Kelch-like protein 13
Weight
73.787kDa
Alternative Names
BTB and kelch domain containing 2; BTB and kelch domain-containing protein 2; kelch-like 13 (Drosophila); kelch-like protein 13; KIAA1309; MGC74791
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Klhl13, check out the Klhl13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Klhl13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLHL13 Monoclonal Antibody (A12505)
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No publications found for A12505
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-KLHL13 Monoclonal Antibody
Question
Will A12505 anti-KLHL13 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
As indicated on the product datasheet, A12505 anti-KLHL13 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-23
Question
We are currently using anti-KLHL13 Monoclonal antibody A12505 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
The anti-KLHL13 Monoclonal antibody (A12505) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
Question
Can you help my question with product A12505, anti-KLHL13 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12505 anti-KLHL13 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-20
Question
Will anti-KLHL13 Monoclonal antibody A12505 work for WB with testis?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
According to the expression profile of testis, KLHL13 is highly expressed in testis. So, it is likely that anti-KLHL13 Monoclonal antibody A12505 will work for WB with testis.
Boster Scientific Support
Answered: 2017-09-21
Question
Do you have a BSA free version of anti-KLHL13 Monoclonal antibody A12505 available?
L. Thomas
Verified customer
Asked: 2016-12-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KLHL13 Monoclonal antibody A12505 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-KLHL13 Monoclonal antibody A12505. Let me know if you need anything else.
D. Miller
Verified customer
Asked: 2014-12-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-15
