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Product Info Summary
| SKU: | A02739-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Klk7 Antibody Picoband™
View all Kallikrein 7 Antibodies
SKU/Catalog Number
A02739-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Klk7 Antibody Picoband™ catalog # A02739-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Klk7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02739-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Klk7 recombinant protein (Position: K30-N238).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02739-1 is reactive to KLK7 in Rat
Applications
A02739-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
27.525kDa
Background of Kallikrein 7
Kallikrein-related peptidase 7 (KLK7) is a serine protease that in humans is encoded by the KLK7 gene. KLK7 is a chymotrypsin-like serine protease of the member of peptidase S1 family. It is synthesized with a signal peptide of 22 amino acids (aa 1-22) and a propeptide region (aa 23-29) that are subsequently cleaved to generate the mature form. Human KLK7 displays about 40 to 60% amino acid homology with other members of the family. It cleaves proteins with aromatic side chains in the P1 position. KLK7 is abundantly expressed in the skin and is also present in the brain, mammary gland, cerebellum, spinal cord, and kidney. Lower levels of expression have been reported in in salivary glands, uterus, thymus, thyroid, placenta, trachea, and testis. Its levels are shown to be up-regulated in ovarian carcinoma, especially late-stage serous carcinoma. Less differentiated tumors of more advanced stage show higher level KLK7 expression. KLK7 activity is inhibited by zinc and copper ions
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Rat
Direct ELISA, 0.1-0.5 µg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Klk7 using anti-Klk7 antibody (A02739-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Klk7 antigen affinity purified polyclonal antibody (Catalog # A02739-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Klk7 at approximately 38 kDa. The expected band size for Klk7 is at 28 kDa.
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Figure 2. IHC analysis of Klk7 using anti-Klk7 antibody (A02739-1).
Klk7 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Klk7 Antibody (A02739-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KLK7 (Source: Uniprot.org, NCBI)
Gene Name
KLK7
Full Name
Kallikrein-7
Weight
27.525kDa
Superfamily
peptidase S1 family
Alternative Names
EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); Kallikrein 7; kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme KLK7 PRSS6, SCCE, hK7 kallikrein related peptidase 7 kallikrein-7|kallikrein 7 (chymotryptic, stratum corneum)|serine protease 6|signal protein|stratum corneum chymotryptic enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK7, check out the KLK7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Klk7 Antibody Picoband™ (A02739-1)
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No publications found for A02739-1
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