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Product Info Summary
| SKU: | A33934-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-KLRA1 KLRA1P Antibody
SKU/Catalog Number
A33934-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KLRA1 KLRA1P Antibody (Catalog # A33934-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KLRA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KLRA1 KLRA1P Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33934-1)
Host
Rabbit
Contents
KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1. The immunogen is located within amino acids 140 - 190 of KLRA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
Reactive Species
A33934-1 is reactive to in Human, Mouse, Rat
Applications
A33934-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
31311 MW
Background of
KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KLRA1 in mouse spleen tissue lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.
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Immunocytochemistry of KLRA1 in 293 cells with KLRA1 antibody at 4 μg/mL.
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Immunofluorescence of KLRA1 in 293 cells with KLRA1 antibody at 20 μg/mL.
Specific Publications For Anti-KLRA1 KLRA1P Antibody (A33934-1)
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Customer Reviews
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