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Product Info Summary
| SKU: | A33282 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-KLRA2 Antibody
SKU/Catalog Number
A33282
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KLRA2 Antibody (Catalog # A33282). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
KLRA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KLRA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33282)
Host
Rabbit
Contents
KLRA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLRA2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of mouse KLRA2. The immunogen is located within the last 50 amino acids of KLRA2.
Reactive Species
A33282 is reactive to KLRA2 in Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.6 kDa
Background of KLRA2
KLRA2 (also known as Ly49B) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA2 is thought to be an inhibitory receptor and associates with the protein tyrosine phosphatases SHP1, SHP2, and SHIP in transfected cells. This antibody is predicted to not cross-react with other LY49 receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33282 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KLRA2 antibody can be used for detection of KLRA2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KLRA2 in mouse spleen tissue lysate with KLRA2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of KLRA2 in mouse spleen tissue with KLRA2 antibody at 2.5 μg/mL.
Specific Publications For Anti-KLRA2 Antibody (A33282)
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