Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | A04175-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-KLRC1 Antibody (C-term)
SKU/Catalog Number
A04175-2
Size
50 µl
Description
Boster Bio Anti-KLRC1 Antibody (C-term) (Catalog # A04175-2). Tested in WB, IHC-P, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KLRC1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04175-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1.
Reactive Species
A04175-2 is reactive to KLRC1 in Human
Calculated molecular weight
26.3 kDa
Background of KLRC1
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. This protein belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04175-2 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
IHC-P: 1:50-1:100
FC: 1:25
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KLRC1 with A04175-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Click image to see more details
Anti-KLRC1 Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Western blot analysis of KLRC1 Antibody (C-term) (Cat. #A04175-2) in Jurkat cell line lysates (35ug/lane). KLRC1 (arrow) was detected using the purified Pab.
Click image to see more details
All lanes : Anti-KLRC1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Click image to see more details
Overlay histogram showing Jurkat cells stained with A04175-2 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A04175-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-KLRC1 Antibody (C-term) (A04175-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-KLRC1 Antibody (C-term)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-KLRC1 Antibody (C-term)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
