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Product Info Summary
| SKU: | A04175-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-KLRC1 Antibody (C-term)
SKU/Catalog Number
A04175-2
Size
50 µl
Description
Boster Bio Anti-KLRC1 Antibody (C-term) (Catalog # A04175-2). Tested in WB, IHC-P, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KLRC1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04175-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04175-2 is reactive to KLRC1 in Human
Reconstitution
Calculated molecular weight
26314 Da
Background of KLRC1
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. This protein belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04175-2 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
IHC-P: 1:50-1:100
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KLRC1 with A04175-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Anti-KLRC1 Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of KLRC1 Antibody (C-term) (Cat. #A04175-2) in Jurkat cell line lysates (35ug/lane). KLRC1 (arrow) was detected using the purified Pab.
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All lanes : Anti-KLRC1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Overlay histogram showing Jurkat cells stained with A04175-2 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A04175-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For KLRC1 (Source: Uniprot.org, NCBI)
Gene Name
KLRC1
Full Name
NKG2-A/NKG2-B type II integral membrane protein
Weight
26314 Da
Alternative Names
NKG2-A/NKG2-B type II integral membrane protein, CD159 antigen-like family member A, NK cell receptor A, NKG2-A/B-activating NK receptor, CD159a, KLRC1, NKG2A KLRC1 CD159A, NKG2, NKG2A killer cell lectin like receptor C1 NKG2-A/NKG2-B type II integral membrane protein|C-lectin type II protein|CD159 -like family member A|NK cell receptor A|NKG2-1/B activating NK receptor|NKG2-A/B type II integral membrane protein|NKG2-A/B-activating NK receptor|killer cell lectin-like receptor subfamily C, member 1|natural killer cell lectin|natural killer group protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLRC1, check out the KLRC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLRC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLRC1 Antibody (C-term) (A04175-2)
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