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- Table of Contents
KREMEN2 antibody (A11875) performs well in ELISA, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunocytochemistry Validation of KREMEN2 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (A11875) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (A11875) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Anti-Kremen protein 2 KREMEN2 Antibody
KREMEN2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL; ICC: 5 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Kremen protein 2 KREMEN2 Antibody
View all KREMEN2 Antibodies
View all KREMEN2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
KREMEN2 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
KREMEN2 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-KREMEN2 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2. The immunogen is located within amino acids 390-440 of KREMEN2.
Cross-Reactivity
Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
48.8 kDa
Background of KREMEN2
Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
Cite This Product
Anti-Kremen protein 2 KREMEN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11875)
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Product Promise
We promise Anti-Kremen protein 2 KREMEN2 Antibody performs excellently in relevant applications for ELISA, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A11875
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026