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Product Info Summary
| SKU: | A09147 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Krueppel-like factor 17 KLF17 Antibody
SKU/Catalog Number
A09147
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Krueppel-like factor 17 KLF17 Antibody (Catalog # A09147). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KLF17 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Krueppel-like factor 17 KLF17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09147)
Host
Rabbit
Contents
KLF17 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17. The immunogen is located within the last 50 amino acids of KLF17.
Cross-reactivity
KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist; this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins.
Reactive Species
A09147 is reactive to KLF17 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.6 kDa
Background of KLF17
KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09147 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of KLF17 in mouse liver tissue with KLG17 antibody at 5 μg/ml.
Specific Publications For Anti-Krueppel-like factor 17 KLF17 Antibody (A09147)
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