Product Info Summary
SKU: | A00120-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Krueppel-like factor 4 KLF4 Antibody
SKU/Catalog Number
A00120-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Krueppel-like factor 4 KLF4 Antibody catalog # A00120-1. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 4 KLF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00120-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human IKKbeta around the phosphorylation site of Y199.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00120-1 is reactive to KLF4 in Human, Mouse, Rat
Applications
A00120-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.671kDa
Background of KLF4
Participates in DNA repair and in chromosomal DNA replication.
Kesti T., J. Biol. Chem. 268:10238-10245(1993).
Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of KLF4 using Anti-Krueppel-like factor 4 KLF4 Antibody (A00120-1).
ICC staining KLF4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Krueppel-like factor 4 KLF4 Antibody A00120-1
Western blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54.671kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut) KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Krueppel-like factor 4 KLF4 Antibody (A00120-1)
Hello CJ!
A00120-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
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