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Product Info Summary
| SKU: | M01275 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ku80 XRCC5 Rabbit Monoclonal Antibody
View all Ku80/XRCC5 Antibodies
SKU/Catalog Number
M01275
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ku80 XRCC5 Rabbit Monoclonal Antibody catalog # M01275. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ku80 XRCC5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01275)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FAE-24
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ku80
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01275 is reactive to XRCC5 in Human
Applications
M01275 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
82.705kDa
Background of Ku80/XRCC5
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using Ku80 Antibody.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Western blot analysis of Ku80 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)
Gene Name
XRCC5
Full Name
X-ray repair cross-complementing protein 5
Weight
82.705kDa
Superfamily
ku80 family
Alternative Names
80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; Ku80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku autoantigen protein p86|nuclear factor IV|thyroid-lupus autoantigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC5, check out the XRCC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ku80 XRCC5 Rabbit Monoclonal Antibody (M01275)
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No publications found for M01275
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Ku80 XRCC5 Rabbit Monoclonal Antibody
Question
My question regarding product M01275, anti-Ku80 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01275 anti-Ku80 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-14
Question
Is a blocking peptide available for product anti-Ku80 Rabbit Monoclonal antibody (M01275)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do provide the blocking peptide for product anti-Ku80 Rabbit Monoclonal antibody (M01275). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-31
Question
We bought anti-Ku80 Rabbit Monoclonal antibody for WB on cervix carcinoma in the past. I am using human, and We are going to use the antibody for IF next. We want examining cervix carcinoma as well as heart osteoblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
I have checked the website and datasheets of our anti-Ku80 Rabbit Monoclonal antibody and I see that M01275 has been tested on human in both WB and IF. Thus M01275 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-24
Question
I was wanting to use your anti-Ku80 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
You can see on the product datasheet, M01275 anti-Ku80 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-04
Question
We are interested in using your anti-Ku80 Rabbit Monoclonal antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
R. Parker
Verified customer
Asked: 2019-08-14
Answer
Thanks for your inquiry. This M01275 anti-Ku80 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-14
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-Ku80 Rabbit Monoclonal antibody M01275. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
We are currently using anti-Ku80 Rabbit Monoclonal antibody M01275 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The anti-Ku80 Rabbit Monoclonal antibody (M01275) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-29
Question
Our lab were happy with the WB result of your anti-Ku80 Rabbit Monoclonal antibody. However we have observed positive staining in kidney nucleus. using this antibody. Is that expected? Could you tell me where is XRCC5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
From what I have seen in literature, kidney does express XRCC5. Generally XRCC5 expresses in nucleus. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-11
Question
We have seen staining in human neuroblastoma. Are there any suggestions? Is anti-Ku80 Rabbit Monoclonal antibody supposed to stain neuroblastoma positively?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Based on literature neuroblastoma does express XRCC5. Based on Uniprot.org, XRCC5 is expressed in kidney, neuroblastoma, coronary artery, thyroid uterus, cervix carcinoma, heart osteoblast, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-07-30
Question
Does anti-Ku80 Rabbit Monoclonal antibody M01275 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
According to the expression profile of cervix carcinoma, XRCC5 is highly expressed in cervix carcinoma. So, it is likely that anti-Ku80 Rabbit Monoclonal antibody M01275 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-06
Question
Is this M01275 anti-Ku80 Rabbit Monoclonal antibody reactive to the isotypes of XRCC5?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
The immunogen of M01275 anti-Ku80 Rabbit Monoclonal antibody is A synthesized peptide derived from human Ku80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Ku80 Rabbit Monoclonal antibody M01275. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-04
Question
Does M01275 anti-Ku80 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Anderson
Verified customer
Asked: 2017-02-20
Answer
It shows on the product datasheet, M01275 anti-Ku80 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-20
Question
Do you have a BSA free version of anti-Ku80 Rabbit Monoclonal antibody M01275 available?
D. Williams
Verified customer
Asked: 2016-07-29
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Ku80 Rabbit Monoclonal antibody M01275 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-29
Question
I am interested in to test anti-Ku80 Rabbit Monoclonal antibody M01275 on human cervix carcinoma for research purposes, then I may be interested in using anti-Ku80 Rabbit Monoclonal antibody M01275 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Gonzalez
Verified customer
Asked: 2014-09-23
Answer
The products we sell, including anti-Ku80 Rabbit Monoclonal antibody M01275, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-09-23
Question
I see that the anti-Ku80 Rabbit Monoclonal antibody M01275 works with WB, what is the protocol used to produce the result images on the product page?
K. Johnson
Verified customer
Asked: 2013-01-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-01-07
