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Product Info Summary
| SKU: | A00715 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Laforin EPM2A Antibody
SKU/Catalog Number
A00715
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Laforin EPM2A Antibody (Catalog # A00715). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EPM2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Laforin EPM2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00715)
Host
Rabbit
Contents
EPM2A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EPM2A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human EPM2A. The immunogen is located within amino acids 190 - 240 of EPM2A.
Cross-reactivity
At least four isoforms of EPM2A are known to exist; this antibody will detect all but the shortest isoform.
Reactive Species
A00715 is reactive to EPM2A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
37.2 kDa
Background of EPM2A
The Epilepsy, progressive myoclonus type 2A protein (EPM2A) is a dual-specificity phosphatase that associates with polyribosomes. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. EPM2A interacts with a number of proteins known to be involved in glycogen metabolism and has been shown to have robust phosphatase activity against a phosphorylated complex carbohydrate, suggesting that EPM2A may be involved in the regulation of glycogen metabolism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00715 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EPM2A antibody can be used for detection of EPM2A by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SW480 in SW480 cell lysate with EPM2A antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of EPM2A in SW480 cells with EPM2A antibody at 2.5 μg/ml.
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Immunofluorescence of EPM2A in SW480 cells with EPM2A antibody at 5 μg/ml.
Specific Publications For Anti-Laforin EPM2A Antibody (A00715)
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