Anti-LAG3 Monoclonal Antibody [2G8]

Product Name

Anti-LAG3 Monoclonal Antibody [2G8]

View all LAG-3 Antibodies

SKU/Catalog Number

M02869-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LAG3 Monoclonal Antibody [2G8] (Catalog # M02869-1). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

LAG-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LAG3 Monoclonal Antibody [2G8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02869-1)

Host

Mouse

Contents

LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 2G8

Isotype

IgG1,k

Immunogen

LAG-3 antibody was raised against the extracellular domain of human LAG-3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

M02869-1 is reactive to LAG3 in Human

Applications

M02869-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

57.449kDa

Background of LAG-3

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LAG-3 antibody can be used for ELISA starting at 0.25 μg/mL. For Western blot start at 0.25 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 1 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.

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Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

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Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.

Green: LAG3 Antibody [2G8] (RF16082)
Blue: DAPI staining

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Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.

Red: LAG3 Antibody [2G8] (RF16082)
Blue: DAPI staining

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Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.

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Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

Gene/Protein Information For LAG3 (Source: Uniprot.org, NCBI)

Alternative Names

17b4 lag3; 17b4 neutralizing; 17b4; anti human lag3; LAG-3; CD223; CD223 Antigen; CD223 antigen; CD223; FDC; Human LAG3; Human LAG-3; LAG-3 17b4; LAG-3 blocking; LAG3; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC LAG3 CD223 lymphocyte activating 3 lymphocyte activation gene 3 protein|lymphocyte-activation gene 3

For more info on LAG3, check out the LAG3 Infographic

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In this infographic, you will see the following information for LAG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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