Anti-Laminin subunit gamma-2 LAMC2 Antibody

Product Name

Anti-Laminin subunit gamma-2 LAMC2 Antibody

View all LAMC2 Antibodies

SKU/Catalog Number

PA1582

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Laminin subunit gamma-2 LAMC2 Antibody catalog # PA1582. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Laminin subunit gamma-2 LAMC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1582)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LAMC2, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1582 is reactive to LAMC2 in Human, Mouse, Rat

Applications

PA1582 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

131 kDa

Calculated molecular weight

130.976kDa

Background of LAMC2

Laminin gamma2, Laminin subunit gamma-2, is a protein that in humans is encoded by the LAMC2 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly though to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat

Validation Images & Assay Conditions

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Figure 1.Western blot analysis of LAMC2 using anti-LAMC2 antibody (PA1582).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
Lane 3: human PC-3 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat lung tissue lysates.
Lane 6: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMC2 antigen affinity purified polyclonal antibody (Catalog # PA1582) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMC2 at approximately 131KD. The expected band size for LAMC2 is at 131KD.

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Figure 2. IHC analysisof LAMC2 using anti-LAMC2 antibody (PA1582).
LAMC2 was detected in paraffin-embedded section of human lung cancertissue. Heat mediated antigen retrieval was performed inEDTA buffer (pH8.0, epitope retrieval solution). Thetissue section was blocked with 10% goat serum. The tissue sectionwasthen incubated with 1μg/ml rabbit anti-LAMC2 Antibody (PA1582) overnight at 4°C. Biotinylated goat anti-rabbitIgG was used assecondary antibody and incubated for 30 minutes at 37°C. The tissuesection was developed using Strepavidin-Biotin-Complex (SABC)(Catalog# SA1022) with DAB as the chromogen.

Gene/Protein Information For LAMC2 (Source: Uniprot.org, NCBI)

Alternative Names

BM600; BM600-100kDa; Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; CSF; EBR2; EBR2A; Epiligrin subunit gamma; kalinin (105kD); Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; kalinin-105kDa; ladsin (140kDa); Ladsin 140 kDa subunit; LAMB2Tlaminin, gamma 2 (nicein (100kD), kalinin (105kD), BM600 (100kD), Herlitzjunctional epidermolysis bullosa)); Laminin B2t chain; laminin subunit gamma-2; laminin, gamma 2; Laminin-5 subunit gamma; LAMNB2B2T; Large adhesive scatter factor 140 kDa subunit; MGC138491; MGC141938; nicein (100kDa); Nicein subunit gamma; nicein-100kDa LAMC2 B2T, BM600, CSF, EBR2, EBR2A, LAMB2T, LAMNB2 laminin subunit gamma 2 laminin subunit gamma-2|BM600-100kDa|CSF 140 kDa subunit|cell-scattering factor 140 kDa subunit|epiligrin subunit gamma|kalinin subunit gamma|ladsin 140 kDa subunit|laminin B2t chain|laminin, gamma 2|large adhesive scatter factor 140 kDa subunit|nicein subunit gamma

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In this infographic, you will see the following information for LAMC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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