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Product Info Summary
| SKU: | PB9142 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
SKU/Catalog Number
PB9142
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® catalog # PB9142. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9142)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9142 is reactive to Lamc1 in Human
Observed Molecular Weight
177 kDa
Calculated molecular weight
177.3 kDa
Background of Lamc1
Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9142 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Positive Control
WB: human A431 whole cell, human A549 whole cell, human PC-3 whole cell, human Caco-2 whole cellrat NRK whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse Hepa1-6 whole cell
IHC: human lung cancer tissue
IHC-F: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of Laminin using anti-Laminin antibody (PB9142).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # PB9142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 220-250 kDa. The expected band size for Laminin is at 177 kDa.
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IHC analysis of Laminin using anti-Laminin antibody (PB9142).
Laminin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Laminin using anti-Laminin antibody (PB9142).
Laminin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (PB9142)
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1 Customer Q&As for Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
Question
I was wanting to use using your anti-Laminin/Lamc1/Lamc2/Lamc3 antibody for hemidesmosome assembly studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you for your inquiry. This PB9142 anti-Laminin/Lamc1/Lamc2/Lamc3 antibody is tested on hela whole cell lysate, hepg2 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC-P, IHC-F, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-04
