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- Table of Contents
Lamc1 antibody (PB9142) performs well in IHC, IHC-F, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of Laminin using anti-Laminin antibody (PB9142).
Laminin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Laminin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
Lamc1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, IHC-F, WB |
Tested Applications
Lamc1 antibody was positively detected by WB in:
human A431 whole cell, human A549 whole cell, human PC-3 whole cell, human Caco-2 whole cellrat NRK whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse Hepa1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Lamc1 antibody was positively detected by IHC in:
human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Lamc1 antibody was positively detected by IHC-F in:
human placenta tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
View all LAMC1 Antibodies
View all LAMC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
177 kDa
Calculated Molecular Weight
177.3 kDa
Background of Lamc1
Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
Cite This Product
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9142)
Protocols
Western Blot protocol for Lamc1 antibody
Immunohistochemistry (IHC) protocol for Lamc1 antibody
Publications
Application:
Species:
Anti-fibrotic effects of puerarin on CCl4-induced hepatic fibrosis in rats possibly through the regulation of PPAR-γ expression and inhibition of PI3K/Akt pathway
Published: 2013-03-13
Journal: FOOD AND CHEMICAL TOXICOLOGY
Impact Factor: 3.01
PMID: 23500774
Biocompatibility of pure titanium modified by human endothelial cell-derived extracellular matrix
Published: 2010-01-22
Journal: APPLIED SURFACE SCIENCE
Application: IF
Impact Factor: 1.62
Species: Human
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Product Promise
We promise Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® performs excellently in relevant applications for IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9142
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026