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Product Info Summary
| SKU: | M00780-4 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | African green monkey, Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-LAMP1 Antibody
SKU/Catalog Number
M00780-4
Size
80 µl
Description
Boster Bio Anti-LAMP1 Antibody (Catalog # M00780-4). Tested in WB, IHC-P, IF application(s). This antibody reacts with Human, African green monkey.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-LAMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780-4)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1483CT517.45.23
Isotype
IgG1,k
Immunogen
This LAMP1 antibody is generated from a mouse immunized with a recombinant protein.
Reactive Species
M00780-4 is reactive to LAMP1 in African green monkey, Human
Calculated molecular weight
44.9 kDa
Background of LAMP1
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00780-4 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling LAMP1 with M00780-4 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-LAMP1 Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: Jurkat whole cell lysates
Lane 4: HT-1080 whole cell lysates
Lane 5: COS-7 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00780-4 staining LAMP1 in human kidney sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-LAMP1 Antibody (M00780-4)
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