Anti-LASS5 CERS5 Antibody

Product Name

Anti-LASS5 CERS5 Antibody

View all CERS5 Antibodies

SKU/Catalog Number

A08612-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LASS5 CERS5 Antibody (Catalog # A08612-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LASS5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LASS5 CERS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08612-1)

Host

Rabbit

Contents

LASS5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LASS5 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human LASS5. The immunogen is located within amino acids 90 - 140 of LASS5.

Cross-reactivity

Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.

Reactive Species

A08612-1 is reactive to CERS5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.8 kDa

Background of CERS5

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08612-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LASS5 antibody can be used for detection of LASS5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CERS5

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