Anti-LIM domain-binding protein 1 LDB1 Antibody

Product Name

Anti-LIM domain-binding protein 1 LDB1 Antibody

View all LDB1 Antibodies

SKU/Catalog Number

A01977

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-LIM domain-binding protein 1 LDB1 Antibody (Catalog # A01977). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-LIM domain-binding protein 1 LDB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01977)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01977 is reactive to LDB1 in Human

Applications

A01977 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

46.533kDa

Background of LDB1

LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:425,000
ChIP: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of LDB1 expression in Jurkat whole cell lysate (lane 1). LDB1 was detected using rabbit anti-LDB1 Antigen Affinity purified polyclonal antibody (Catalog # A01977) at 1:1800. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For LDB1 (Source: Uniprot.org, NCBI)

Alternative Names

carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; CLIM2hLdb1; LDB-1; LIM domain binding 1; LIM domain-binding factor-1; LIM domain-binding protein 1; NLILIM domain-binding factor CLIM2; Nuclear LIM interactor LDB1 CLIM-2, CLIM2, LDB-1, NLI LIM domain binding 1 LIM domain-binding protein 1|LIM domain-binding factor CLIM2|LIM domain-binding factor-1|carboxy terminal LIM domain protein 2|carboxyl-terminal LIM domain-binding protein 2|nuclear LIM interactor

For more info on LDB1, check out the LDB1 Infographic

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In this infographic, you will see the following information for LDB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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