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Product Info Summary
| SKU: | A01960 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-LEDGF PSIP1 Monoclonal Antibody
SKU/Catalog Number
A01960
Size
100μl
Form
Liquid
Description
Boster Bio Anti-LEDGF PSIP1 Monoclonal Antibody catalog # A01960. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LEDGF PSIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01960)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
11168
Isotype
IgG
Immunogen
Purified recombinant fragment of human LEDGF expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01960 is reactive to PSIP1 in Human, Monkey, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
60.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01960 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using LEDGF Monoclonal Antibody.
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Immunofluorescence analysis of NIH/3T3 cells using LEDGF Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot analysis using LEDGF Monoclonal Antibody against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), HeLa (6), and NIH/3T3 (7) cell lysate.
Specific Publications For Anti-LEDGF PSIP1 Monoclonal Antibody (A01960)
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Customer Q&As
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6 Customer Q&As for Anti-LEDGF PSIP1 Monoclonal Antibody
Question
Does anti-LEDGF Monoclonal antibody A01960 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
According to the expression profile of leukemic t-cell, PSIP1 is highly expressed in leukemic t-cell. So, it is likely that anti-LEDGF Monoclonal antibody A01960 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-LEDGF Monoclonal antibody A01960 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, monkey, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The anti-LEDGF Monoclonal antibody (A01960) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-16
Question
Can you help my question with product A01960, anti-LEDGF Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01960 anti-LEDGF Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-19
Question
Do you have a BSA free version of anti-LEDGF Monoclonal antibody A01960 available?
J. Li
Verified customer
Asked: 2018-06-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LEDGF Monoclonal antibody A01960 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-19
Question
I am looking for to test anti-LEDGF Monoclonal antibody A01960 on rat leukemic t-cell for research purposes, then I may be interested in using anti-LEDGF Monoclonal antibody A01960 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Rodriguez
Verified customer
Asked: 2018-06-06
Answer
The products we sell, including anti-LEDGF Monoclonal antibody A01960, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-06
Question
I was wanting to use your anti-LEDGF Monoclonal antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
C. Li
Verified customer
Asked: 2014-12-18
Answer
It shows on the product datasheet, A01960 anti-LEDGF Monoclonal antibody has been tested for IF, IHC, WB on human, monkey, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-18
