Anti-LEFTY A LEFTY2 Monoclonal Antibody

LEFTY2 antibody

Boster Bio Anti-LEFTY A LEFTY2 Monoclonal Antibody (Catalog # M06558). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M06558
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: ELISA, WB

Product Name

Anti-LEFTY A LEFTY2 Monoclonal Antibody

View all LEFTY2 Antibodies

SKU/Catalog Number

M06558

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-LEFTY A LEFTY2 Monoclonal Antibody (Catalog # M06558). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-LEFTY A LEFTY2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06558)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 7C5G1H6H10 IgG2a kappa

Isotype

IgG2a kappa

Immunogen

A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06558 is reactive to LEFTY2 in Human

Applications

M06558 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

40.92kDa

Background of LEFTY2

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000
WB: 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For LEFTY2 (Source: Uniprot.org, NCBI)

Gene Name

LEFTY2

Full Name

Left-right determination factor 2

Weight

40.92kDa

Superfamily

TGF-beta family

Alternative Names

Ebaf; Endometrial Bleeding-Associated Factor; LEFTA; Left-Right Determination Factor 2; Left-right determination factor A; Lefty-2; LEFTYA; Protein Lefty-2; Protein Lefty-A; TGFB4; TGF-beta 4; TGF-beta-4; Transforming Growth Factor Beta-4 LEFTY2 EBAF, LEFTA, LEFTYA, TGFB4 left-right determination factor 2 left-right determination factor 2|TGF-beta-4|endometrial bleeding-associated factor|left-right determination factor A|protein lefty-2|protein lefty-A|transforming growth factor beta-4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LEFTY2, check out the LEFTY2 Infographic

LEFTY2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LEFTY2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-LEFTY A LEFTY2 Monoclonal Antibody

Question

We are currently using anti-LEFTY A Monoclonal antibody M06558 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

The anti-LEFTY A Monoclonal antibody (M06558) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-19

Question

I was wanting to use your anti-LEFTY A Monoclonal antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?

J. Johnson

Verified customer

Asked: 2019-06-05

Answer

You can see on the product datasheet, M06558 anti-LEFTY A Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-05

Question

you antibody to test anti-LEFTY A Monoclonal antibody M06558 on human uterus for research purposes, then I may be interested in using anti-LEFTY A Monoclonal antibody M06558 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

The products we sell, including anti-LEFTY A Monoclonal antibody M06558, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-10-30

Order DetailsPrice
M06558

100 μg

$612

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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