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Product Info Summary
| SKU: | M06558 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LEFTY A LEFTY2 Monoclonal Antibody
SKU/Catalog Number
M06558
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-LEFTY A LEFTY2 Monoclonal Antibody (Catalog # M06558). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-LEFTY A LEFTY2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06558)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 7C5G1H6H10 IgG2a kappa
Isotype
IgG2a kappa
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Reactive Species
M06558 is reactive to LEFTY2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
40.9 kDa
Background of LEFTY2
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06558 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000
WB: 1:2,000
This antibody has been tested by ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.
Validation Images & Assay Conditions
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Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. Detection occurs after 1.0 µg of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1,000. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-LEFTY A LEFTY2 Monoclonal Antibody (M06558)
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3 Customer Q&As for Anti-LEFTY A LEFTY2 Monoclonal Antibody
Question
We are currently using anti-LEFTY A Monoclonal antibody M06558 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The anti-LEFTY A Monoclonal antibody (M06558) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use your anti-LEFTY A Monoclonal antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
J. Johnson
Verified customer
Asked: 2019-06-05
Answer
You can see on the product datasheet, M06558 anti-LEFTY A Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
you antibody to test anti-LEFTY A Monoclonal antibody M06558 on human uterus for research purposes, then I may be interested in using anti-LEFTY A Monoclonal antibody M06558 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
The products we sell, including anti-LEFTY A Monoclonal antibody M06558, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-30
