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Product Info Summary
| SKU: | A16257 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LEMD1 Antibody
SKU/Catalog Number
A16257
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LEMD1 Antibody (Catalog # A16257). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
LEMD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LEMD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16257)
Host
Rabbit
Contents
LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1. The immunogen is located within amino acids 90 - 140 of LEMD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
Reactive Species
A16257 is reactive to LEMD1 in Human, Mouse
Applications
A16257 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
20.326kDa
Background of LEMD1
LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Protein Target Info & Infographic
Gene/Protein Information For LEMD1 (Source: Uniprot.org, NCBI)
Gene Name
LEMD1
Full Name
LEM domain-containing protein 1
Weight
20.326kDa
Alternative Names
cancer/testis antigen 50; LEM domain containing 1; LEM domain-containing protein 1; LEMP-1 LEMD1 CT50, LEMP-1 LEM domain containing 1 LEM domain-containing protein 1|LEM domain protein 1|cancer/testis antigen 50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEMD1, check out the LEMD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEMD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LEMD1 Antibody (A16257)
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