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Product Info Summary
| SKU: | A09079 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-LEMD2 Antibody
SKU/Catalog Number
A09079
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LEMD2 Antibody (Catalog # A09079). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LEMD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09079)
Host
Rabbit
Contents
LEMD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2. The immunogen is located within amino acids 220 - 270 of LEMD2.
Cross-reactivity
At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform.
Reactive Species
A09079 is reactive to LEMD2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
57.0 kDa
Background of LEMD2
The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09079 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LEMD2 in 293 cell lysate with LEMD2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of LEMD2 in 3T3 cells with LEMD2 antibody at 2.5 μg/mL.
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Immunofluorescence of LEMD2 in 3T3 cells with LEMD2 antibody at 20 μg/mL.
Specific Publications For Anti-LEMD2 Antibody (A09079)
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