Click image to see more details
Product Info Summary
| SKU: | M00350 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody
SKU/Catalog Number
M00350
BM5122 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody catalog # M00350. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00350)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABDO-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Leptin Receptor
Reactive Species
M00350 is reactive to LEPR in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
132.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00350 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human SH-SY5Y whole cell, human K562 whole cell, human HEL whole cell, rat kidney tissue, rat spleen tissue, mouse kidney tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of Leptin Receptor using anti-Leptin Receptor antibody (M00350).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin Receptor antigen affinity purified monoclonal antibody (M00350) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin Receptor at approximately 150 kDa. The expected band size for Leptin Receptor is at 132 kDa.
Specific Publications For Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody (M00350)
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Customer Q&As
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6 Customer Q&As for Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for plasma using anti-Leptin Receptor Rabbit Monoclonal antibody M00350. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Would anti-Leptin Receptor Rabbit Monoclonal antibody M00350 work on dog WB with fetal liver?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Our lab technicians have not tested anti-Leptin Receptor Rabbit Monoclonal antibody M00350 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Leptin Receptor Rabbit Monoclonal antibody M00350 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog fetal liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-04
Question
Does anti-Leptin Receptor Rabbit Monoclonal antibody M00350 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
According to the expression profile of plasma, LEPR is highly expressed in plasma. So, it is likely that anti-Leptin Receptor Rabbit Monoclonal antibody M00350 will work for WB with plasma.
Boster Scientific Support
Answered: 2019-06-11
Question
Do you have a BSA free version of anti-Leptin Receptor Rabbit Monoclonal antibody M00350 available?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Leptin Receptor Rabbit Monoclonal antibody M00350 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-15
Question
Does M00350 anti-Leptin Receptor Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
It shows on the product datasheet, M00350 anti-Leptin Receptor Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Leptin Receptor Rabbit Monoclonal antibody M00350. Let me know if you need anything else.
S. Jha
Verified customer
Asked: 2015-03-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-16
