Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody

Leptin R antibody

Boster Bio Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody catalog # M00350. Tested in WB application. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00350
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody

View all Leptin R Antibodies

SKU/Catalog Number

M00350

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody catalog # M00350. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00350)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABDO-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Leptin Receptor

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00350 is reactive to LEPR in Human, Mouse, Rat

Applications

M00350 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

132.494kDa

Background of Leptin R

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For LEPR (Source: Uniprot.org, NCBI)

Gene Name

LEPR

Full Name

Leptin receptor

Weight

132.494kDa

Superfamily

type I cytokine receptor family

Alternative Names

B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295 LEPR CD295, LEP-RD, OB-R, OBR, LEPR leptin receptor leptin receptor|OB receptor|huB219

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LEPR, check out the LEPR Infographic

LEPR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LEPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00350 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Srinivasan G,Parida S,Pavithra S, Panigrahi M,Sahoo M,Singh TU,Madhu CL,Manickam K,Shyamkumar TS,Kumar D,Mishra SK.Leptin receptor stimulation in late pregnant mouse uterine tissue inhibits spontaneous contractions by increasing NO and cGMP.Cytokine.2021
Species: Mouse
M00350 usage in article: APP:WB, SAMPLE:UTERINE TISSUE, DILUTION:1:300

The Influence of LepR Tyrosine Site Mutations on Mouse Ovary Development and Related Gene Expression Changes

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6 Customer Q&As for Anti-Leptin Receptor LEPR Rabbit Monoclonal Antibody

Question

Here is the WB image, lot number and protocol we used for plasma using anti-Leptin Receptor Rabbit Monoclonal antibody M00350. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-30

Question

Would anti-Leptin Receptor Rabbit Monoclonal antibody M00350 work on dog WB with fetal liver?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

Our lab technicians have not tested anti-Leptin Receptor Rabbit Monoclonal antibody M00350 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Leptin Receptor Rabbit Monoclonal antibody M00350 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog fetal liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-04

Question

Does anti-Leptin Receptor Rabbit Monoclonal antibody M00350 work for WB with plasma?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

According to the expression profile of plasma, LEPR is highly expressed in plasma. So, it is likely that anti-Leptin Receptor Rabbit Monoclonal antibody M00350 will work for WB with plasma.

Boster Scientific Support

Answered: 2019-06-11

Question

Do you have a BSA free version of anti-Leptin Receptor Rabbit Monoclonal antibody M00350 available?

Verified Customer

Verified customer

Asked: 2019-04-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Leptin Receptor Rabbit Monoclonal antibody M00350 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-15

Question

Does M00350 anti-Leptin Receptor Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-17

Answer

It shows on the product datasheet, M00350 anti-Leptin Receptor Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-17

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Leptin Receptor Rabbit Monoclonal antibody M00350. Let me know if you need anything else.

S. Jha

Verified customer

Asked: 2015-03-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-03-16

Order DetailsPrice
M00350

100μL

$370
M00350-30ul

30μL sample

$99
M00350-carrier-free

Carrier Free

$370

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