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Product Info Summary
| SKU: | A05092 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody
SKU/Catalog Number
A05092
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (Catalog # A05092). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
LNPEP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05092)
Host
Rabbit
Contents
LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP. The immunogen is located within amino acids 900 - 950 of LNPEP.
Cross-reactivity
At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
Reactive Species
A05092 is reactive to LNPEP in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
117.3 kDa
Background of LNPEP
LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05092 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of LNPEP in human lung tissue with LNPEP antibody at 20 μg/mL.
Specific Publications For Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (A05092)
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