Anti-LIGHT/Tnfsf14 Antibody Picoband®

Tnfsf14 antibody

Boster Bio Anti-LIGHT/Tnfsf14 Antibody Picoband® catalog # A03516-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03516-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-LIGHT/Tnfsf14 Antibody Picoband®

View all Tnfsf14 Antibodies

SKU/Catalog Number

A03516-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LIGHT/Tnfsf14 Antibody Picoband® catalog # A03516-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LIGHT/Tnfsf14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03516-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse LIGHT recombinant protein (Position: L59-V239).

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03516-1 is reactive to Tnfsf14 in Mouse, Rat

Observed Molecular Weight

26 kDa

Calculated molecular weight

26.3 kDa

Background of Tnfsf14

Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03516-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: mouse spleen tissue, mouse cardiac muscle tissue

Validation Images & Assay Conditions

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5 Customer Q&As for Anti-LIGHT/Tnfsf14 Antibody Picoband®

Question

Does A03516-1 anti-LIGHT/Tnfsf14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

You can see on the product datasheet, A03516-1 anti-LIGHT/Tnfsf14 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-05

Question

I see that the anti-LIGHT/Tnfsf14 antibody A03516-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com

Boster Scientific Support

Answered: 2019-08-16

Question

Is a blocking peptide available for product anti-LIGHT/Tnfsf14 antibody (A03516-1)?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

We do provide the blocking peptide for product anti-LIGHT/Tnfsf14 antibody (A03516-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.

Boster Scientific Support

Answered: 2017-12-04

Question

We are currently using anti-LIGHT/Tnfsf14 antibody A03516-1 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-02

Answer

The anti-LIGHT/Tnfsf14 antibody (A03516-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-02

Question

Will anti-LIGHT/Tnfsf14 antibody A03516-1 work on monkey ELISA with right lobe of liver?

A. Thomas

Verified customer

Asked: 2017-11-02

Answer

Our lab technicians have not tested anti-LIGHT/Tnfsf14 antibody A03516-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-LIGHT/Tnfsf14 antibody A03516-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right lobe of liver in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-02

Order DetailsPrice
A03516-1

100μg

$370
A03516-1-Fluoro488

100 μg Fluoro488 conjugated (liquid)

$570
A03516-1-Fluoro550

100 μg Fluoro550 conjugated (liquid)

$570
A03516-1-Fluoro594

100 μg Fluoro594 conjugated (liquid)

$570
A03516-1-Fluoro647

100 μg Fluoro647 conjugated (liquid)

$670
A03516-1-APC

100 μg APC conjugated (liquid)

$820
A03516-1-FITC

100 μg FITC conjugated (liquid)

$570
A03516-1-Cy3

100 μg Cy3 conjugated (liquid)

$570
A03516-1-Biotin

100 μg Biotin conjugated (liquid)

$570
A03516-1-HRP

100 μg HRP conjugated (liquid)

$570
A03516-1-PE

100 μg PE conjugated (liquid)

$820
A03516-1-carrier-free

Carrier Free

$370
Rainbow Button View conjugates

Free Secondary Antibody

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$55.00  $110.00
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A03516-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 5, 2026