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Product Info Summary
| SKU: | A05231 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LIMA1 Antibody
SKU/Catalog Number
A05231
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LIMA1 Antibody (Catalog # A05231). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-LIMA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05231)
Host
Rabbit
Contents
LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.
Cross-reactivity
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Reactive Species
A05231 is reactive to LIMA1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.2 kDa
Background of LIMA1
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05231 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.
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Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.
Specific Publications For Anti-LIMA1 Antibody (A05231)
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