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LIMA1 antibody (A05231) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.
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Anti-LIMA1 Antibody
LIMA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-LIMA1 Antibody
View all LIMA1 Antibodies
View all LIMA1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
LIMA1 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
LIMA1 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.
Cross-Reactivity
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
85.2 kDa
Background of LIMA1
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
Cite This Product
Anti-LIMA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05231)
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Product Promise
We promise Anti-LIMA1 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05231
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026