Anti-LIMP2 SCARB2 Antibody

Product Name

Anti-LIMP2 SCARB2 Antibody

View all SCARB2 Antibodies

SKU/Catalog Number

A05090

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LIMP2 SCARB2 Antibody (Catalog # A05090). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LIMP2 SCARB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05090)

Host

Rabbit

Contents

LIMP2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LIMP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human LIMP2. The immunogen is located within amino acids 70 - 120 of LIMP2.

Reactive Species

A05090 is reactive to SCARB2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

54.3 kDa

Background of SCARB2

The lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind beta-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted beta-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05090 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LIMP2 antibody can be used for detection of LIMP2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SCARB2

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