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- Table of Contents
LIN28A antibody (A01966-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of PC-3 cells using anti-LIN28A antibody (A01966-2).
Overlay histogram showing PC-3 cells stained with A01966-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN28A Antibody (A01966-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing PC-3 cells stained with A01966-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN28A Antibody (A01966-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Lin28/LIN28A Antibody Picoband®
LIN28A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
LIN28A antibody was positively detected by WB in:
human placenta tissue, human K562 whole cell, human Caco-2 whole cell, rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LIN28A antibody was positively detected by IHC in:
human testis cancer tissue, mouse intestine tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LIN28A antibody was positively detected by IF in:
rat intestine tissue, human mammary cancer tissue
LIN28A antibody was positively detected by FC in:
PC-3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
2μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Lin28/LIN28A Antibody Picoband®
View all LIN28A Antibodies
View all LIN28A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Lin28/LIN28A recombinant protein (Position: S3-N209).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
22.7 kDa
Background of LIN28A
Protein lin-28 homolog A is a protein that in humans is encoded by the LIN28A gene. It is mapped to 1p36.11. This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues.
Cite This Product
Anti-Lin28/LIN28A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01966-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
98%
High Homology
Mouse
97%
High Homology
Rabbit
96%
High Homology
Sheep
95%
High Homology
Dog
95%
High Homology
Cow
94%
High Homology
Zebrafish
68%
Low homology
Drosophila
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LIN28A antibody
Immunohistochemistry (IHC) protocol for LIN28A antibody
Immunofluorescence (IF) protocol for LIN28A antibody
Flow Cytometry (FC) protocol for LIN28A antibody
Publications
Application:
Species:
Lin28/let-7/Bcl-xL pathway: The underlying mechanism of drug resistance in Hep3B cells
Published: 2014-06-25
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.19
Species: Human
PMID: 24970027
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Product Promise
We promise Anti-Lin28/LIN28A Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01966-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026