Anti-LIN9 Antibody Picoband™

Product Name

Anti-LIN9 Antibody Picoband™

View all Lin9 Antibodies

SKU/Catalog Number

A06333-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LIN9 Antibody Picoband™ catalog # A06333-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LIN9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06333-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human LIN9 recombinant protein (Position: F127-D542).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06333-1 is reactive to Lin9 in Human

Applications

A06333-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

61.759kDa

Background of Lin9

Lin-9 homolog is a protein that is encoded by the LIN9 gene in humans. This gene encodes a tumor suppressor protein that inhibits DNA synthesis and oncogenic transformation through association with the retinoblastoma 1 protein. The encoded protein also interacts with a complex of other cell cycle regulators to repress cell cycle-dependent gene expression in non-dividing cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LIN9 using anti-LIN9 antibody (A06333-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN9 antigen affinity purified polyclonal antibody (Catalog # A06333-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN9 at approximately 65 kDa. The expected band size for LIN9 is at 62 kDa.

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Figure 2. IF analysis of LIN9 using anti-LIN9 antibody (A06333-1) and anti-Beta Tubulin antibody (M01857-3).
LIN9 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LIN9 Antibody (A06333-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Lin9 (Source: Uniprot.org, NCBI)

Alternative Names

BARA; BARPsv; hLin-9; huLin-9; lin-9 homolog (C. elegans); Lin-9; pRB-associated protein; protein lin-9 homolog; rb related pathway actor; TGS1; TGSBeta subunit-associated regulator of apoptosis; TUDOR gene similar protein; Type I interferon receptor beta chain-associated protein Lin9|2700022J23Rik, Bara, Lin-9, TGS, TGS1, mLin-9|lin-9 homolog (C. elegans)|protein lin-9 homolog|TUDOR gene similar 1 protein|type I interferon receptor beta chain-associated protein

For more info on Lin9, check out the Lin9 Infographic

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In this infographic, you will see the following information for Lin9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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