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Product Info Summary
| SKU: | PB9610 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Lipocalin 2/LCN2 Antibody Picoband®
SKU/Catalog Number
PB9610
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lipocalin 2/LCN2 Antibody Picoband® catalog # PB9610. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipocalin 2/LCN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9610)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Lipocalin 2 recombinant protein (Position: Q21-G198). Human Lipocalin 2 shares 62 % and 64.4 % amino acid (aa) sequence identity with mouse and rat Lipocalin 2, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9610 is reactive to LCN2 in Human
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.6 kDa
Background of LCN2
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9610 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Whole Cell, SMMC Whole Cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: A549 Whole Cell Lysate at 40ug,
Lane 2: SMMC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9610) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 kDa. The expected band size for Lipocalin 2 is at 22 kDa.
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IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610).
Lipocalin 2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lipocalin 2 Antibody (PB9610) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Lipocalin 2/LCN2 Antibody Picoband® (PB9610)
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Customer Q&As
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2 Customer Q&As for Anti-Lipocalin 2/LCN2 Antibody Picoband®
Question
What is the formulation for PB10059?
Verified customer
Asked: 2020-10-22
Answer
The Anti-Lipocalin 2/LCN2 Antibody Picoband™ (PB9610) contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Boster Scientific Support
Answered: 2020-10-22
Question
We are currently using anti-Lipocalin 2/LCN2 antibody PB9610 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
M. Patel
Verified customer
Asked: 2016-06-20
Answer
The anti-Lipocalin 2/LCN2 antibody (PB9610) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-20
