Human Lipocalin-2/NGAL ELISA Kit PicoKine™

Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 10pg/ml. Cited in 17 publication(s).

Product Info Summary

SKU: EK0853
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Product Name

Human Lipocalin-2/NGAL ELISA Kit PicoKine™

See all Lipocalin-2/NGAL products

SKU/Catalog Number

EK0853

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0853)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-G198

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0853 is reactive to LCN2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Application Guarantee

EK0853 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0853-CAP Anti-Human LCN2 Pre-coated 96-well strip microplate 1
EK0853-ST Human LCN2 Standard 2 vials, 10 ng/tube
EK0853-DA Human LCN2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0780.2020.4100.5470.8310.9531.4091.885

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Lipocalin-2/NGAL, Dilution ratio of 1:100, concentration in serum is 40-100ng/ml.

Some articles we found to cite concentrations of Lipocalin-2/NGAL in samples: 24528623 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2561510542225415375138
Standard deviation19.281.54281.9419.0584.53323.69
CV (%)7.5%5.4%5.2%7.5%5.5%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1256230223260242166.6%
Sample 215101658154618071630115.727%
Sample 354225479536247305248302.055.7%
*number of samples for each test n=16.

Gene/Protein Information For LCN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P80188

Gene ID

3934

Gene Name

LCN2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.588kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LCN2, check out the LCN2 Infographic

LCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0853 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

AB0166 The Effect of a Treatment with Allogenic Mesenchimal Stromal Cells (MSCS) on Urinary Biomarkers in a Mouse Model of Systemic Lupus Erythematosus
Species: Mouse

Sirvan Atashak,Stephen R. Stannard,Ali Daraei et al.High-Intensity Interval Training Improves Lipocalin-2 and Omentin-1 Levels in Men with Obesity,18 February 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-254389
Species: Human
EK0853 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Zhang H,Che L,Wang Y,Zhou H,Gong H,Man X,Zhao Q.Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes.Int Urol Nephrol.2021 Jan 28.doi:10.1007/s1
Species: Human
EK0853 usage in article: APP:ELISA, SAMPLE:URINE, DILUTION:NA

Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome

Endothelial Glycocalyx Damage Is Associated with Leptospirosis Acute Kidney Injury

Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis

Neutrophil gelatinase-associated lipocalin reflects the severity of anemia without iron deficiency and secondary hyperparathyroidism in hemodialysis patients

Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients

Association between neutrophil geletinase-associated lipocalin and iron deficiency anemia in children on chronic dialysis

Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Sch?nlein Purpura Patients with Renal Involvement

Urine and serum neutrophil gelatinase-associated lipocalin cut-off point for the prediction of acute kidney injury

Benli E, Ayyildiz SN, Cirrik S, Noyan T, Ayyildiz A, Cirakoglu A. Int Braz J Urol. 2017 Sep-Oct;43(5):887-895. doi: 10.1590/S1677-5538.IBJU.2016.0638. Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP ...

Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...

Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...

Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...

Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

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9 Customer Q&As for Human Lipocalin-2/NGAL ELISA Kit PicoKine™

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Lipocalin-2 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-05-21

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Lipocalin-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-05-21

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

V. Jackson

Verified customer

Asked: 2019-10-23

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-10-23

Question

Q: What is the optimal O.D. value for Lipocalin-2 ELISA kit? I used your Lipocalin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Lipocalin-2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lipocalin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lipocalin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-07-22

Question

Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?

S. Roberts

Verified customer

Asked: 2019-05-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-05-23

Question

Q: how to analyze ELISA data? I have performed Lipocalin-2 level in serum.

Verified Customer

Verified customer

Asked: 2019-05-18

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-18

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-03-06

Question

Q: can I use citrate plasma as samples in Human Lipocalin-2 Picokine® ELISA Kit (Catalog # EK0853)?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Lipocalin-2 causing disruption of its protein structure. Lipocalin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Lipocalin-2 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-08-21

Question

Q: which procedure should I follow in order to thaw whole blood sample for Lipocalin-2 ELISA after freezing?

Z. Jones

Verified customer

Asked: 2018-04-19

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Lipocalin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-04-19

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