- Table of Content
Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine.. Sensitivity: 10pg/ml. Cited in 16 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum, plasma (heparin) , saliva and urine.|
|Sample Volume:||100μl per well|
|Assay Range:||156 pg/ml - 10,000 pg/ml|
|Product Name||Human Lipocalin-2/NGAL ELISA Kit PicoKine™
See more Lipocalin-2/NGAL products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine.. Sensitivity: 10pg/ml.|
|Cite This Product||Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0853)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum, plasma (heparin) , saliva and urine.|
|Assay Range||156 pg/ml - 10,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: Q21-G198|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||3934|
|Species Of This Entry||Human|
|Protein Name||Neutrophil gelatinase-associated lipocalin|
|Alternative Names||Lipocalin-2/NGAL|24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0853-CAP||Anti-Human LCN2 Pre-coated 96-well strip microplate||1|
|EK0853-ST||Human LCN2 Standard||2 vials, 10 ng/tube|
|EK0853-DA||Human LCN2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0853 Human Lipocalin-2/NGAL ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Lipocalin-2/NGAL ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Sirvan Atashak,Stephen R. Stannard,Ali Daraei et al.High-Intensity Interval Training Improves Lipocalin-2 and Omentin-1 Levels in Men with Obesity,18 February 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-254389
EK0853 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
|Zhang H,Che L,Wang Y,Zhou H,Gong H,Man X,Zhao Q.Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes.Int Urol Nephrol.2021 Jan 28.doi:10.1007/s1
EK0853 usage in article: APP:ELISA, SAMPLE:URINE, DILUTION:NA
|PubMed ID 33511504|
|Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome||PubMed ID 25757454|
|Endothelial Glycocalyx Damage Is Associated with Leptospirosis Acute Kidney Injury||PubMed ID 25624405|
|Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis||PubMed ID 29784944|
|Neutrophil gelatinase-associated lipocalin reflects the severity of anemia without iron deficiency and secondary hyperparathyroidism in hemodialysis patients||PubMed ID 28752141|
|Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients||PubMed ID 27114982|
|Association between neutrophil geletinase-associated lipocalin and iron deficiency anemia in children on chronic dialysis||PubMed ID 25364361|
|Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Sch?nlein Purpura Patients with Renal Involvement||PubMed ID 24963810|
|Urine and serum neutrophil gelatinase-associated lipocalin cut-off point for the prediction of acute kidney injury||PubMed ID 24627874|
|Benli E, Ayyildiz SN, Cirrik S, Noyan T, Ayyildiz A, Cirakoglu A. Int Braz J Urol. 2017 Sep-Oct;43(5):887-895. doi: 10.1590/S1677-5538.IBJU.2016.0638. Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP ...||PubMed ID 28792192|
|Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...||PubMed ID 26047940|
|Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...||PubMed ID 23476127|
|Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...||PubMed ID 23837035|
|Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...||PubMed ID 24324637|
|Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.||PubMed ID 24955162|
Domestically in USA and Canada, ships next business day if available in stock.
Comprehensive info on LCN2, beautifully presented.Open Infographic
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
We promise all of our products perform as described in datasheets.