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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin) , saliva and urine.|
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Human Lipocalin-2/NGAL ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0853)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q21-G198
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0853 is reactive to LCN2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) , saliva and urine.
EK0853 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0853-CAP||Anti-Human LCN2 Pre-coated 96-well strip microplate||1|
|EK0853-ST||Human LCN2 Standard||2 vials, 10 ng/tube|
|EK0853-DA||Human LCN2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Lipocalin-2/NGAL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lipocalin-2/NGAL, Dilution ratio of 1:100, concentration in serum is 40-100ng/ml.
Some articles we found to cite concentrations of Lipocalin-2/NGAL in samples: 24528623 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LCN2 (Source: Uniprot.Org, NCBI)
Neutrophil gelatinase-associated lipocalin
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LCN2, check out the LCN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0853 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
AB0166 The Effect of a Treatment with Allogenic Mesenchimal Stromal Cells (MSCS) on Urinary Biomarkers in a Mouse Model of Systemic Lupus Erythematosus
Sirvan Atashak,Stephen R. Stannard,Ali Daraei et al.High-Intensity Interval Training Improves Lipocalin-2 and Omentin-1 Levels in Men with Obesity,18 February 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-254389
EK0853 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhang H,Che L,Wang Y,Zhou H,Gong H,Man X,Zhao Q.Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes.Int Urol Nephrol.2021 Jan 28.doi:10.1007/s1
EK0853 usage in article: APP:ELISA, SAMPLE:URINE, DILUTION:NA
Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome
Endothelial Glycocalyx Damage Is Associated with Leptospirosis Acute Kidney Injury
Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis
Neutrophil gelatinase-associated lipocalin reflects the severity of anemia without iron deficiency and secondary hyperparathyroidism in hemodialysis patients
Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients
Association between neutrophil geletinase-associated lipocalin and iron deficiency anemia in children on chronic dialysis
Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Sch?nlein Purpura Patients with Renal Involvement
Urine and serum neutrophil gelatinase-associated lipocalin cut-off point for the prediction of acute kidney injury
Benli E, Ayyildiz SN, Cirrik S, Noyan T, Ayyildiz A, Cirakoglu A. Int Braz J Urol. 2017 Sep-Oct;43(5):887-895. doi: 10.1590/S1677-5538.IBJU.2016.0638. Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP ...
Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...
Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...
Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...
Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
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