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Product Info Summary
| SKU: | A00217 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LKB1 STK11 Antibody
SKU/Catalog Number
A00217
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LKB1 STK11 Antibody (Catalog # A00217). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
LKB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LKB1 STK11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00217)
Host
Rabbit
Contents
LKB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LKB1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human LKB1. The immunogen is located within amino acids 310 - 360 of LKB1.
Reactive Species
A00217 is reactive to STK11 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.6 kDa
Background of STK11
The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00217 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
Specific Publications For Anti-LKB1 STK11 Antibody (A00217)
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