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Product Info Summary
| SKU: | A03296 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LMX1B Antibody
SKU/Catalog Number
A03296
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LMX1B Antibody (Catalog # A03296). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LMX1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LMX1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03296)
Host
Rabbit
Contents
LMX1B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B. The immunogen is located within amino acids 280 - 330 of LMX1B.
Cross-reactivity
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Reactive Species
A03296 is reactive to LMX1B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
44.9 kDa
Background of LMX1B
The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03296 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-LMX1B Antibody (A03296)
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