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Product Info Summary
| SKU: | A07965 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-LPAAT- theta GPAT3 Antibody
SKU/Catalog Number
A07965
Size
100μl
Form
Liquid
Description
Boster Bio Anti-LPAAT- theta GPAT3 Antibody catalog # A07965. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LPAAT- theta GPAT3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07965)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human AGPAT9. AA range:381-430
Reactive Species
A07965 is reactive to GPAT3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
48.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07965 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using PLCH Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, COLO205 cells, HeLa cells, and HUVEC cells, using PLCH Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
Specific Publications For Anti-LPAAT- theta GPAT3 Antibody (A07965)
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Customer Q&As
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7 Customer Q&As for Anti-LPAAT- theta GPAT3 Antibody
Question
Can you help my question with product A07965, anti-LPAAT- theta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07965 anti-LPAAT- theta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
Will anti-LPAAT- theta antibody A07965 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
According to the expression profile of cervix carcinoma, GPAT3 is highly expressed in cervix carcinoma. So, it is likely that anti-LPAAT- theta antibody A07965 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-12-19
Question
I was wanting to use your anti-LPAAT- theta antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
As indicated on the product datasheet, A07965 anti-LPAAT- theta antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-30
Question
Do you have a BSA free version of anti-LPAAT- theta antibody A07965 available?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LPAAT- theta antibody A07965 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-LPAAT- theta antibody A07965. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-22
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-LPAAT- theta antibody A07965. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-03
Question
Does anti-LPAAT- theta antibody A07965 work on bovine WB with pns testis?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
Our lab technicians have not tested anti-LPAAT- theta antibody A07965 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-LPAAT- theta antibody A07965 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine pns testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-26
