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Product Info Summary
| SKU: | A02467 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LPIN1 Antibody
SKU/Catalog Number
A02467
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LPIN1 Antibody (Catalog # A02467). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LPIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-LPIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02467)
Host
Rabbit
Contents
LPIN Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LPIN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human LPIN1. The immunogen is located within the last 50 amino acids of LPIN1.
Cross-reactivity
LPIN1 antibody is human and mouse reactive. At least four isoforms of LPIN1 are known to exist.
Reactive Species
A02467 is reactive to LPIN1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
98.7 kDa
Background of LPIN1
LPIN1, also known as PAP1, is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol (reviewed in 1). LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism (1,2). Mutations in LPIN1 are associated with metabolic diseases such as type 2 diabetes and recurrent acute myoglobinuria (3,4) and it is also a candidate for several human lipodystrophy syndromes (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02467 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LPIN1 antibody can be used for detection of LPIN1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.
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Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.
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Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.
Specific Publications For Anti-LPIN1 Antibody (A02467)
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