Anti-LRAT Antibody

Product Name

Anti-LRAT Antibody

View all LRAT Antibodies

SKU/Catalog Number

A03362

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-LRAT Antibody (Catalog # A03362). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LRAT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03362)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human LRAT.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03362 is reactive to LRAT in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

25703 MW

Background of LRAT

Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.

Ruiz A., J. Biol. Chem. 274:3834-3841(1999).
Mondal M.S., Biochemistry 39:5215-5220(2000).
Thompson D.A., Nat. Genet. 28:123-124(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A03362 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For LRAT (Source: Uniprot.org, NCBI)

Alternative Names

Lecithin retinol acyltransferase;2.3.1.135;Phosphatidylcholine--retinol O-acyltransferase;LRAT; LRAT LCA14 lecithin retinol acyltransferase lecithin retinol acyltransferase|lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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