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Product Info Summary
| SKU: | A16557 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-LRRC45 Antibody (C-term)
SKU/Catalog Number
A16557
Size
80 µl
Description
Boster Bio Anti-LRRC45 Antibody (C-term) (Catalog # A16557). Tested in WB, IF, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-LRRC45 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16557)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
Reactive Species
A16557 is reactive to LRRC45 in Human
Calculated molecular weight
76.0 kDa
Background of LRRC45
ZNF90 may be involved in transcriptional regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16557 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Immunofluorescent analysis of U251 cells, using LRRC45 Antibody (C-term). A16557 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
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Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody (C-term). A16557 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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LRRC45 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRRC45 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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LRRC45 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-LRRC45 Antibody (C-term) (A16557)
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