Product Info Summary
SKU: | A12074-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LRRN1 Antibody Picoband™
View all LRRN1/NLRR-1 Antibodies
SKU/Catalog Number
A12074-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRRN1 Antibody Picoband™ catalog # A12074-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LRRN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12074-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human LRRN1 recombinant protein (Position: T74-R655).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A12074-1 is reactive to Lrrn1 in Human, Mouse, Rat
Applications
A12074-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
80.548kDa
Background of LRRN1/NLRR-1
LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26-631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94-384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LRRN1 using anti-LRRN1 antibody (A12074-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN1 antigen affinity purified polyclonal antibody (Catalog # A12074-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRN1 at approximately 95 kDa. The expected band size for LRRN1 is at 81 kDa.
Click image to see more details
Figure 2. IHC analysis of LRRN1 using anti-LRRN1 antibody (A12074-1).
LRRN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LRRN1 Antibody (A12074-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-LRRN1 antibody (A12074-1).
Overlay histogram showing SH-SY5Y cells stained with A12074-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRN1 Antibody (A12074-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Lrrn1 (Source: Uniprot.org, NCBI)
Gene Name
Lrrn1
Full Name
Leucine-rich repeat neuronal protein 1
Weight
80.548kDa
Alternative Names
fibronectin type III, immunoglobulin and leucine rich repeat domains 3; FIGLER3; KIAA1497; leucine rich repeat neuronal 1; leucine-rich repeat neuronal protein 1; LRN; LRRN1; Neuronal leucine-rich repeat protein 1; NLRR1; NLRR-1 Lrrn1|2810047E21Rik, NL, NLRR-1|leucine rich repeat protein 1, neuronal|leucine-rich repeat neuronal protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lrrn1, check out the Lrrn1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lrrn1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRN1 Antibody Picoband™ (A12074-1)
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No publications found for A12074-1
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