Product Info Summary
| SKU: | A13110 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LRRTM4 Antibody
SKU/Catalog Number
A13110
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRRTM4 Antibody (Catalog # A13110). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
LRRTM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LRRTM4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13110)
Host
Rabbit
Contents
LRRTM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LRRTM4 antibody was raised against a 16 amino acid synthetic peptide near the center of human LRRTM4. The immunogen is located within amino acids 350 - 400 of LRRTM4.
Cross-reactivity
At least two isoforms of LRRTM4 are known to exist; this antibody will both isoforms. LRRTM4 antibody is predicted to not cross-react with other LRRTM family members.
Reactive Species
A13110 is reactive to LRRTM4 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.2 kDa
Background of LRRTM4
The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Little is known about the function of LRRTM4; in mouse, it is expressed in the limb mesenchyme, neural tube, caudal mesoderm and in three distinct regions of the head. Later expression occurs in a subset of the developing sclerotome. Its similarity to other LRRTM family members suggests that LRRTM4 may also play a role in the development and maintenance of the vertebrate nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13110 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LRRTM4 antibody can be used for detection of LRRTM4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LRRTM4 in HeLa cell lysate with LRRTM4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of LRRTM4 in human brain tissue with LRRTM4 antibody at 2.5 μg/mL.
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Immunofluorescence of LRRTM4 in human brain tissue with LRRTM4 antibody at 20 μg/mL.
Specific Publications For Anti-LRRTM4 Antibody (A13110)
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