Anti-Ltb Antibody Picoband™

Product Name

Anti-Ltb Antibody Picoband™

View all Lymphotoxin beta/TNFSF3 Antibodies

SKU/Catalog Number

A02565-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ltb Antibody Picoband™ catalog # A02565-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ltb Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02565-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived mouse Ltb recombinant protein (Position: R68-G306).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02565-2 is reactive to LTB in Mouse, Rat

Applications

A02565-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

52588 MW

Background of Lymphotoxin beta/TNFSF3

Lymphotoxin-beta (LT-beta) also known as tumor necrosis factor C (TNF-C) is a protein that in humans is encoded by the LTB gene. Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ltb using anti-Ltb antibody (A02565-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse Raw264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ltb antigen affinity purified polyclonal antibody (Catalog # A02565-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ltb at approximately 25 kDa. The expected band size for Ltb is at 32 kDa.

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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-Ltb antibody (A02565-2).
Overlay histogram showing RAW264.7 cells stained with A02565-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ltb Antibody (A02565-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For LTB (Source: Uniprot.org, NCBI)

Alternative Names

LTB; lymphotoxin beta (TNF superfamily, member 3); Lymphotoxin beta; lymphotoxin-beta; p33; TNFCTNF-C; TNFSF3; TNFSF3LT-beta; Tumor necrosis factor C; Tumor necrosis factor ligand superfamily member 3 LTB TNFC, TNFSF3, TNLG1C, p33 lymphotoxin beta lymphotoxin-beta|LT-beta|TNF superfamily member 3|TNF-C|lymphotoxin beta (TNF superfamily, member 3)|tumor necrosis factor C|tumor necrosis factor ligand 1C|tumor necrosis factor ligand superfamily member 3

For more info on LTB, check out the LTB Infographic

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In this infographic, you will see the following information for LTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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