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Product Info Summary
| SKU: | A09496-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LY6E/SCA-2 Antibody Picoband®
SKU/Catalog Number
A09496-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LY6E/SCA-2 Antibody Picoband® catalog # A09496-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LY6E/SCA-2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09496-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LY6E/SCA-2 recombinant protein (Position: L21-S101).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09496-2 is reactive to LY6E in Human
Observed Molecular Weight
14 kDa
Calculated molecular weight
13.5 kDa
Background of LY6E
Lymphocyte antigen 6E is a protein that in humans is encoded by the LY6E gene. Increased expression of Ly6E is associated with poor survival outcome in multiple malignancies as determined by a survey of more than 130 published clinical studies of gene expression studies on cancer tissue samples and adjacent normal tissues. Ly6E is associated with drug resistance and tumor immune escape in breast cancer. Further research is required to validate Ly6E for translation research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09496-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human A549 whole cell
IHC: human placenta tissue
FCM: SiHa cell
Validation Images & Assay Conditions
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Western blot analysis of LY6E/SCA-2 using anti-LY6E/SCA-2 antibody (A09496-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6E/SCA-2 antigen affinity purified polyclonal antibody (Catalog # A09496-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6E/SCA-2 at approximately 14 kDa. The expected band size for LY6E/SCA-2 is at 14 kDa.
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IHC analysis of LY6E/SCA-2 using anti-LY6E/SCA-2 antibody (A09496-2).
LY6E/SCA-2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LY6E/SCA-2 Antibody (A09496-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Flow Cytometry analysis of SiHa cells using anti-LY6E/SCA-2 antibody (A09496-2).
Overlay histogram showing SiHa cells stained with A09496-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LY6E/SCA-2 Antibody (A09496-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-LY6E/SCA-2 Antibody Picoband® (A09496-2)
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1 Customer Q&As for Anti-LY6E/SCA-2 Antibody Picoband®
Question
What is the isotype of A09496-2? Is it rabbit IgG?
Verified customer
Asked: 2022-05-16
Answer
The isotype of the Anti-LY6E/SCA-2 Antibody Picoband™ (A09496-2) is rabbit IgG.
Boster Scientific Support
Answered: 2022-05-17
