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Product Info Summary
| SKU: | M04060 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LYRIC Monoclonal Antibody
SKU/Catalog Number
M04060
BM5468 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LYRIC Monoclonal Antibody catalog # M04060. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LYRIC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04060)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADIF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LYRIC Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion.
Reactive Species
M04060 is reactive to MTDH in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
63.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04060 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of LYRIC expression in HeLa cell lysate.
Specific Publications For Anti-LYRIC Monoclonal Antibody (M04060)
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4 Customer Q&As for Anti-LYRIC Monoclonal Antibody
Question
Would M04060 anti-LYRIC Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
You can see on the product datasheet, M04060 anti-LYRIC Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-02
Question
Would anti-LYRIC Monoclonal antibody M04060 work for WB with leukemic t-cell?
P. Huang
Verified customer
Asked: 2018-12-04
Answer
According to the expression profile of leukemic t-cell, MTDH is highly expressed in leukemic t-cell. So, it is likely that anti-LYRIC Monoclonal antibody M04060 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-12-04
Question
I was wanting to use your anti-LYRIC Monoclonal antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
W. Krishna
Verified customer
Asked: 2015-08-05
Answer
You can see on the product datasheet, M04060 anti-LYRIC Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-05
Question
Is this M04060 anti-LYRIC Monoclonal antibody reactive to the isotypes of MTDH?
L. Singh
Verified customer
Asked: 2013-03-08
Answer
The immunogen of M04060 anti-LYRIC Monoclonal antibody is A synthesized peptide derived from human LYRIC Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-08
