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Product Info Summary
| SKU: | M04027 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LYVE1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04027
BM4905 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LYVE1 Rabbit Monoclonal Antibody catalog # M04027. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LYVE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04027)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOCO-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LYVE1
Reactive Species
M04027 is reactive to LYVE1 in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
35.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04027 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human A549 whole cell, human K562 whole cell, human HepG2 whole cell, human HEL whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Western blot analysis of LYVE1 using anti-LYVE1 antibody (M04027).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYVE1 antigen affinity purified monoclonal antibody (Catalog # M04027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYVE1 at approximately 29 kDa. The expected band size for LYVE1 is at 35 kDa.
Specific Publications For Anti-LYVE1 Rabbit Monoclonal Antibody (M04027)
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Customer Q&As
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7 Customer Q&As for Anti-LYVE1 Rabbit Monoclonal Antibody
Question
Can M04027 be used for IHC?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
M04027 cannot be used for IHC.
Boster Scientific Support
Answered: 2020-04-28
Question
Does M04027 anti-LYVE1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
You can see on the product datasheet, M04027 anti-LYVE1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-24
Question
Here is the WB image, lot number and protocol we used for esophagus using anti-LYVE1 Rabbit Monoclonal antibody M04027. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
Is this M04027 anti-LYVE1 Rabbit Monoclonal antibody reactive to the isotypes of LYVE1?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The immunogen of M04027 anti-LYVE1 Rabbit Monoclonal antibody is A synthesized peptide derived from human LYVE1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-10
Question
Would M04027 work on IHC experiments?
Verified customer
Asked: 2019-05-24
Answer
The Anti-LYVE1 Rabbit Monoclonal Antibody (M04027) cannot work with IHC.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-LYVE1 Rabbit Monoclonal antibody M04027 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
The anti-LYVE1 Rabbit Monoclonal antibody (M04027) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-29
Question
May i know is Anti-LYVE1 Rabbit Monoclonal Antibody M04027 can used for IHC, IH testing ? Possible for Bosterbio to try on application above ?
Verified Customer
Verified customer
Asked: 2014-05-20
Answer
This is a sourced product. We didn't verify M04027 with IHC. The customer can run a pilot IHC test.
Boster Scientific Support
Answered: 2014-05-20
