Anti-Mad2L1 Antibody Picoband™

MAD2L1 antibody

Boster Bio Anti-Mad2L1 Antibody Picoband™ catalog # PB9282. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9282
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-Mad2L1 Antibody Picoband™

View all MAD2L1 Antibodies

SKU/Catalog Number

PB9282

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mad2L1 Antibody Picoband™ catalog # PB9282. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mad2L1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9282)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9282 is reactive to MAD2L1 in Human

Applications

PB9282 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.51kDa

Background of MAD2L1

Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MAD2L1 (Source: Uniprot.org, NCBI)

Gene Name

MAD2L1

Full Name

Mitotic spindle assembly checkpoint protein MAD2A

Weight

23.51kDa

Superfamily

MAD2 family

Alternative Names

HSMAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1 (yeast); MAD2L1; MAD2-like protein 1; MAD2mitotic arrest deficient, yeast, homolog-like 1; Mitotic arrest deficient 2-like protein 1; mitotic spindle assembly checkpoint protein MAD2A MAD2L1 HSMAD2, MAD2 mitotic arrest deficient 2 like 1 mitotic spindle assembly checkpoint protein MAD2A|MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2 mitotic arrest deficient-like 1|MAD2-like protein 1|mitotic arrest deficient 2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAD2L1, check out the MAD2L1 Infographic

MAD2L1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAD2L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PB9282

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Mad2L1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Mad2L1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-Mad2L1 Antibody Picoband™

Question

Please see the WB image, lot number and protocol we used for lung using anti-Mad2L1 antibody PB9282. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-21

Question

Would PB9282 anti-Mad2L1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

As indicated on the product datasheet, PB9282 anti-Mad2L1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-11

Question

We are currently using anti-Mad2L1 antibody PB9282 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

The anti-Mad2L1 antibody (PB9282) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-12

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Mad2L1 antibody PB9282. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-05

Question

Do you have a BSA free version of anti-Mad2L1 antibody PB9282 available?

W. Jones

Verified customer

Asked: 2018-01-18

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mad2L1 antibody PB9282 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-18

Order DetailsPrice
PB9282

100μg

$370
PB9282-10ug

10μg sample (liquid)

$99
PB9282-Biotin

100 μg Biotin conjugated

$570
PB9282-Cy3

100 μg Cy3 conjugated

$570
PB9282-Dylight488

100 μg Dylight488 conjugated

$570
PB9282-Dylight550

100 μg Dylight550 conjugated

$570
PB9282-Dylight594

100 μg Dylight594 conjugated

$570
PB9282-FITC

100 μg FITC conjugated

$570
PB9282-HRP

100 μg HRP conjugated

$570
PB9282-APC

100 μg APC conjugated

$670
PB9282-PE

100 μg PE conjugated

$670
PB9282-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9282
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.