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Product Info Summary
| SKU: | RP1066 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor MafA MAFA Antibody Picoband®
SKU/Catalog Number
RP1066
PB0412 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor MafA MAFA Antibody catalog # RP1066. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor MafA MAFA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1066)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MAFA, identical to the related mouse sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1066 is reactive to MAFA in Human, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.0 kDa
Background of MAFA
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1066 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human HEK293 whole cell, human K562 whole cell, human 22RV1 whole cell, rat PC-12 whole cell,
Validation Images & Assay Conditions
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Western blot analysis of MAFA using anti-MAFAantibody (RP1066).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 22RV1 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody (Catalog # RP1066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFA at approximately 37KD. The expected band size for MAFA is at 37KD.
Specific Publications For Anti-Transcription factor MafA MAFA Antibody Picoband® (RP1066)
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Customer Q&As
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2 Customer Q&As for Anti-Transcription factor MafA MAFA Antibody Picoband®
Question
Has the RP1066 middle region been tested in human and mouse samples?
Verified customer
Asked: 2021-02-16
Answer
The immunogen sequence of the Anti-Transcription Factor MafA MAFA Antibody (RP1066) is the middle region of human MAFA (136-167aa EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR). Our lab worked on human, mouse and rat samples and failed to observe the target band in mouse samples.
Boster Scientific Support
Answered: 2021-02-22
Question
We are currently using anti-MAFA antibody RP1066 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
The anti-MAFA antibody (RP1066) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-26
