Product Info Summary
SKU: | A08285 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MAP3K10 Antibody
SKU/Catalog Number
A08285
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MAP3K10 Antibody catalog # A08285. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP3K10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08285)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ANAPC5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08285 is reactive to MAP3K10 in Human, Mouse, Rat
Applications
A08285 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
103.694kDa
Background of MLK2
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Yu H., Science 279:1219-1222(1998).
Bechtel S., BMC Genomics 8:399-399(2007).
Kraft C., EMBO J. 22:6598-6609(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-MAP3K10 Antibody A08285
Western blot (WB) analysis of MAP3K10 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:RAW264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
Lane4:HELA whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAP3K10 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K10
Full Name
Mitogen-activated protein kinase kinase kinase 10
Weight
103.694kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; MAP3K10; MEKK10; mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 kinase; MLK2; MLK2MKN28 derived nonreceptor_type serine/threonine kinase; MST; MSTEC 2.7.11.25; Protein kinase MST MAP3K10 MEKK10, MLK2, MST mitogen-activated protein kinase kinase kinase 10 mitogen-activated protein kinase kinase kinase 10|MKN28 derived nonreceptor_type serine/threonine kinase|MKN28 kinase|mixed lineage kinase 2|protein kinase MST
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K10, check out the MAP3K10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP3K10 Antibody (A08285)
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No publications found for A08285
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Customer Reviews
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