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Product Info Summary
| SKU: | A07188 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAP4K3/Glk Antibody
SKU/Catalog Number
A07188
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MAP4K3/Glk Antibody (Catalog # A07188). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP4K3/Glk Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07188)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human MAP4K3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07188 is reactive to MAP4K3 in Human, Mouse, Rat
Applications
A07188 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
101.316kDa
Background of MAP4K3
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
Diener K., Proc. Natl. Acad. Sci. U.S.A. 94:9687-9692(1997).
Gorry M.C., Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
Edwards J., Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and K562 cells, using MAP4K3 antibody A07188.
Protein Target Info & Infographic
Gene/Protein Information For MAP4K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP4K3
Full Name
Mitogen-activated protein kinase kinase kinase kinase 3
Weight
101.316kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; germinal center kinase-like kinase; Germinal center kinase-related protein kinase; GLKMEKKK 3; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1 MAP4K3 GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1 mitogen-activated protein kinase kinase kinase kinase 3 mitogen-activated protein kinase kinase kinase kinase 3|MAPK/ERK kinase kinase kinase 3|MEK kinase kinase 3|germinal center kinase-like kinase|germinal center kinase-related protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP4K3, check out the MAP4K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP4K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP4K3/Glk Antibody (A07188)
Hello CJ!
No publications found for A07188
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-MAP4K3/Glk Antibody
Question
My question regarding product A07188, anti-MAP4K3/Glk antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07188 anti-MAP4K3/Glk antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-21
Question
See attached the WB image, lot number and protocol we used for testis using anti-MAP4K3/Glk antibody A07188. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
Would anti-MAP4K3/Glk antibody A07188 work on dog WB with kidney?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Our lab technicians have not validated anti-MAP4K3/Glk antibody A07188 on dog. You can run a BLAST between dog and the immunogen sequence of anti-MAP4K3/Glk antibody A07188 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
I was wanting to use your anti-MAP4K3/Glk antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
It shows on the product datasheet, A07188 anti-MAP4K3/Glk antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Is this A07188 anti-MAP4K3/Glk antibody reactive to the isotypes of MAP4K3?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
The immunogen of A07188 anti-MAP4K3/Glk antibody is Synthesized peptide derived from N-terminal of human MAP4K3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-08
Question
I see that the anti-MAP4K3/Glk antibody A07188 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-27
Question
We are currently using anti-MAP4K3/Glk antibody A07188 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
The anti-MAP4K3/Glk antibody (A07188) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-14
