Click image to see more details
Product Info Summary
| SKU: | A30524 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MAP4K6 MINK1 Antibody
SKU/Catalog Number
A30524
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MAP4K6 MINK1 Antibody (Catalog# A30524). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP4K6 MINK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30524)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MAP4K6. Immunogen sequence location: 401-450
Reactive Species
A30524 is reactive to MINK1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
149.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30524 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using MINK1 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from COLO cells, using MAP4K6 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MAP4K6 MINK1 Antibody (A30524)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-MAP4K6 MINK1 Antibody
Question
We are currently using anti-MAP4K6 antibody A30524 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The anti-MAP4K6 antibody (A30524) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-06
Question
Is a blocking peptide available for product anti-MAP4K6 antibody (A30524)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do provide the blocking peptide for product anti-MAP4K6 antibody (A30524). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-23
Question
Is this A30524 anti-MAP4K6 antibody reactive to the isotypes of MINK1?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
The immunogen of A30524 anti-MAP4K6 antibody is A peptide derived from human MAP4K6. Immunogen sequence location: 401-450. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-21
Question
Here is the WB image, lot number and protocol we used for platelet using anti-MAP4K6 antibody A30524. Please let me know if you require anything else.
G. Gonzalez
Verified customer
Asked: 2017-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-MAP4K6 antibody A30524. Let me know if you need anything else.
S. Mangal
Verified customer
Asked: 2013-01-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-31
