Product Info Summary
SKU: | A30663 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MARCH5 MARCH5; Antibody
SKU/Catalog Number
A30663
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MARCH5 MARCH5; Antibody (Catalog# A30663). Tested in IF, ICC, WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARCH5 MARCH5; Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30663)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MARCH5. Immunogen sequence location: 21-70
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30663 is reactive to MARCHF5 in Human, Mouse, Rat
Applications
A30663 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
31.232kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500-2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MARCHF5 (Source: Uniprot.org, NCBI)
Gene Name
MARCHF5
Full Name
E3 ubiquitin-protein ligase MARCHF5
Weight
31.232kDa
Alternative Names
E3 ubiquitin-protein ligase MARCHF5 MARCHF5 MARCH-V, MARCH5, MITOL, RNF153 membrane associated ring-CH-type finger 5 E3 ubiquitin-protein ligase MARCHF5|E3 ubiquitin-protein ligase MARCH5|RING-type E3 ubiquitin transferase MARCH5|RING-type E3 ubiquitin transferase MARCHF5|membrane associated ring finger 5|membrane-associated RING finger protein 5|membrane-associated RING-CH protein V|membrane-associated ring finger (C3HC4) 5|mitochondrial ubiquitin ligase|ring finger protein 153
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARCHF5, check out the MARCHF5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARCHF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARCH5 MARCH5; Antibody (A30663)
Hello CJ!
No publications found for A30663
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MARCH5 MARCH5; Antibody
Question
Our lab want to know about using your anti-MARCH5 antibody for regulation of mitochondrial fission studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
J. Anderson
Verified customer
Asked: 2015-01-30
Answer
We appreciate your inquiry. This A30663 anti-MARCH5 antibody is tested on a549 cells. It is guaranteed to work for IF in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-30