Anti-MATH1/HATH1/ATOH1 Antibody Picoband™

Product Name

Anti-MATH1/HATH1/ATOH1 Antibody Picoband™

View all MATH1 Antibodies

SKU/Catalog Number

A02207-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MATH1/HATH1/ATOH1 Antibody Picoband™ catalog # A02207-1. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MATH1/HATH1/ATOH1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02207-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MATH1/HATH1, different from the related mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02207-1 is reactive to ATOH1 in Human, Rat

Applications

A02207-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

38.16kDa

Background of MATH1

Protein atonal homolog 1 is a protein that in humans is encoded by the ATOH1 gene. This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. ATOH1 is required for the formation of both neural and non-neural cell types. Using genetic deletion in mice, Atoh1 has been shown to be essential for formation of cerebellar granule neurons, inner ear hair cells, spinal cord interneurons, Merkel cells of the skin, and intestinal secretory cells (goblet, enteroendocrine, and Paneth cells).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MATH1/HATH1/ATOH1 using anti-MATH1/HATH1/ATOH1 antibody (A02207-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATH1/HATH1/ATOH1 antigen affinity purified polyclonal antibody (Catalog # A02207-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATH1/HATH1/ATOH1 at approximately 38 kDa. The expected band size for MATH1/HATH1/ATOH1 is at 38 kDa.

Gene/Protein Information For ATOH1 (Source: Uniprot.org, NCBI)

Alternative Names

ATH1protein atonal homolog 1; atonal homolog 1 (Drosophila); BHLHA14; bHLHa14Math1; Class A basic helix-loop-helix protein 14; HATH1; Helix-loop-helix protein hATH-1; MATH-1 ATOH1 ATH1, HATH1, MATH-1, bHLHa14 atonal bHLH transcription factor 1 protein atonal homolog 1|atonal homolog 1|atonal homolog bHLH transcription factor 1|class A basic helix-loop-helix protein 14|helix-loop-helix protein hATH-1

For more info on ATOH1, check out the ATOH1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATOH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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