Click image to see more details
Product Info Summary
| SKU: | M02336 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MBD1 Monoclonal Antibody
SKU/Catalog Number
M02336
BM5213 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MBD1 Monoclonal Antibody catalog # M02336. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02336)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACAI-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MBD1
Reactive Species
M02336 is reactive to MBD1 in Human
Observed Molecular Weight
67, 80 kDa
Calculated molecular weight
66.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02336 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human 293T whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MBD1 using anti-MBD1 antibody (M02336).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified monoclonal antibody (Catalog # M02336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD1 at approximately 67, 80 kDa. The expected band size for MBD1 is at 67 kDa.
Specific Publications For Anti-MBD1 Monoclonal Antibody (M02336)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MBD1 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-MBD1 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-MBD1 Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for testis using anti-MBD1 Monoclonal antibody M02336. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-MBD1 Monoclonal antibody M02336 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
S. Johnson
Verified customer
Asked: 2019-07-11
Answer
The anti-MBD1 Monoclonal antibody (M02336) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-11
Question
Do you have a BSA free version of anti-MBD1 Monoclonal antibody M02336 available?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MBD1 Monoclonal antibody M02336 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-07
